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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:8050-0250
中文名称:
英文名称:TBG 890 FEP; 3/16 X 1/4 50FT
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Preparation of Brain Membrane Fractions
strokes). Never use a polytron.Centrifuge the homogenate at 800 x g for 20 min.Spin resulting supernatant at 16,000 x g for 30 min.Resuspend the pellet in 20 mM HEPES buffer (pH 7.4), rehomogenize and centrifuge at 16,000 x g for 15 min.Pool the pellets
Preparation of Brain Membrane Fractions for Western Blot Analysis
-FEP polymer homogenizer at 900 rpm (12�15 strokes). Never use a polytron. Centrifuge the homogenate at 800 x g for 20 min. Spin resulting supernatant at 16,000 x g for 30 min. Resuspend the pellet in 20 mM HEPES buffer
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