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- F110-NXL-S-200-Q
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：F110-NXL-S-200-Q
中文名称：
英文名称：FC FILTER TIP 200UL SINGLE WRAP STERILE 1M/CS
货期：热销品现货，其他请咨询
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文献和实验Single and Double FISH protocols for Drosophila
); dissolve in sterile H2O, divide into 50 ul aliquots and store at -20oC) . 4. 2mg/ml glycine in PBS. 5. RNA hybridization solution: 50% formamide, 5X SSC, 100 ug/ml heparin, 100 ug/ml sonicated salmon sperm DNA and 0.1% Tween 20). Filter through a 20
A quick RNA mini-prep for Neurospora mycelial cultures
. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel
Southern Hybridization Protocols
3. Leave the wash at 65℃ for 30 min. Repeat.Take out the membrane and dry it on paper towel. Wrap with Saran film & place it in a film cassette. Monitor radioactivity of the membrane to estimate exposure times: >1000 counts: 2 hours; 200-300 counts, 24
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