赛默飞世尔Thermo Fisher SULFURIC AC

SDS-PAGE

from Biorad. Store in fridge.IV. 20% SDS (some people filter)V. 10% w/v ammonium persulfate, make fresh every month and store in fridge. Note: old bottles of dry AP can go bad.VI. N,N,N',N'-tetramethylethylene diamine (TEMED). Buy it and store in fridge.VII

DNA Solutions （DNA分离）

to 100 ml FE (formamide/EDTA): 5:1 (v/v) formamide:50 mM EDTA 10 μl ddH2 O 10 μl 100 mM EDTA 100 μl deionized formamide make fresh 10X Fill-in/Kinase buffer : (500 mM Tris-HCl, pH 7.6, 100 mM MgCl2, 10 mM DTT, and 50 ug/ml BSA in double

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column