赛默飞世尔Thermo Fisher Comb, 1.0mm

The ribonuclease protection assay (RPA)

. Place the samples in a heat block pre-warmed to 90°C. Immediately turn the temperature to 56°C (allowing the temperature to ramp down slowly) and incubate for 12-16 hours. Turn the heat block to 37°C for 15 minutes prior to the RNase treatments

基于PCR技术的染色质沉淀分析

(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR

SDS-PAGE

. However, they give innacurate molecular weights. 9. Samples of E. coli extracts: resuspend the pellet from 1 OD ml of culture in 50 µl sample buffer (1 OD ml= 1 ml of culture at OD600=1). On a BRL gel box with the 0.8 mm thick 20 well comb, load 20 µl of this (this is about 80