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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70105-719-489
中文名称：
英文名称：BTL AMBER GLS B/R 1000ML 12/CS
货期：热销品现货，其他请咨询
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文献和实验Basic procedures for bacteria culture
Protocol 1. Add the following reagents to a 0.5 ml microcentrifuge tube, in the order listed: sterile ddH2O q.s 10X kinase buffer 1 ul DNA x ul [[gamma]]-[32-P]-rATP 10 uCi T4 polynucleotide kinase 1 ul (3U/ul
in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15
contained in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20℃ overnight at this stage.2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes
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