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文献和实验Metabolic labeling Immunoprecipitation
pipet,then completely with narrow-mouth,gel-loading tip attached to P200 pipet so that you won’t take out ProteinG beads. 11.To the slightly moist pellet of beads,add 30µl SDS-PAGE sample buffer (either reducing or nonreducing,depending on the purpose
Metabolic labeling Immunoprecipitation
, then completely with narrow-mouth, gel-loading tip attached to P200 pipet so that you won’t take out ProteinG beads. 11. To the slightly moist pellet of beads,add 30µl SDS-PAGE sample buffer (either reducing or nonreducing, depending on the purpose) 12. Wait
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
. Biophysical Journal . 77, 2813-2826 (1999). 11. Williams, C. et al. Cell sourcing and culture conditions for fibrin-based valve constructs. Tissue engineering . 12, 1489-502 (2006). 12. Grassl, E.D., Oegema, T.R. & Tranquillo, R.T. A fibrin-based
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