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货号：T_701PLCU22T
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英文名称：1000 PPM CU IN 2% HNO3, 250 ML
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文献和实验. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue
Single and Double FISH protocols for Drosophila
in confocal microscopy., ed. Stephen Paddock, Humana Press Inc., in press. 1. MATERIALS 1.1 RNA Probe Preparation 1. 1.5 ml microcentrifuge tubes, autoclaved. 2. RNAse free DEPC treated or double-distilled water. 3. 5X T7/T
. Alternatively, you can wash coverslips by ‘dunking’ 10 times into 4 successive 250ml beakers filled with PBS, but dunk gently. 9. In a humidified chamber, incubate cells with secondary antibody (diluted in 2% BSA in PBS) for 1hr at RT. If co-staining
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