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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310332742
中文名称:
英文名称:7105KH 5.0µL SYR (24/70/3)
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
x dNTP mix: Digoxigenin-11-dUTP pre-mixed with other nucleotides (1 mmol/l dATP, 1 mmol/l dCTP, 1 mmol/l dGTP, 0.65 mmol/l dTTP, 0.35 mmol/l DIG-dUTP). (Boehringer Cat# 1277 065.) - 10x PBS: 80g NaCl, 2g KCl, 6.1g anhydrous Na2HPO4, 2g KH2PO4, H2
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T
Immunohistochemisty-Enzymatic Protocol
Bacitracin (Sigma Catalog # B-0125), pH 7.2 PBS: 50 mM Na2HPO4 and 140 mM NaCl, pH 7.2 Incubation Buffer: 1 bovine serum albumin (Sigma Catalog # A2153), 1 normal donkey serum (Sigma Catalog # D9663), 0.3 Triton X-100 (Sigma Catalog # T9284
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