赛默飞世尔Thermo Fisher 2.0ML SC-MC

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

Southern Hybridization Protocols

8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65

小RNA的Northern BLOT

BP 152-5 (MW 121.14) (890 mM) 110g Boric acid Fisher (electroph.gr) BP 168-500 (MW 61.83) (20 mM) 40 mL 0.5M EDTA pH 8.0 H2O to 2L, check pH w. strip (take out and drizzle ~500 ul on pH strip, ~pH 8.5, filter sterilize 0.2