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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701MP114540022
中文名称：
英文名称：CSM-HIS-LEU-TRP-URA 100 G
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文献和实验Two-hybrid analysis of genetic
combinations of strains,plasmids,and reporters can be used for mating (Section 3).In one common version (Finley and Brent,1994),the strain expressing the bait (bait strain)is RFY206 (MATa ura3-52 his3Æ200 leu2-3 lys2Æ201 trp1::hisG)transformed with the HIS3
Performing a hunt by interaction mating
) on 100-mm Gal/Raff/CM-Ura,-His,-Trp,-Leu plates. Plate 100 µl from tubes 10-4 , 10-5 , and 10-6 on 100-mm Gal/Raff/CM-Ura,-His,-Trp plates. Incubate plates at 30o C. Count the colonies on each plate after 2-5 days. Selecting interactors 20
Solutions for Yeast Complete (YC) Media
Solutions (100X stocks, except URA=10X stock) URA 1 mg/ml ADE 1 mg/ml LYS 10 mg/ml MET 5 mg/ml TRP 10 mg/ml LEU 10 mg/ml HIS 5 mg/ml Autoclave to sterilize, except filter sterilize TRP Store all stocks at 4 deg C, except LEU (store at RT
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