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货号：T_70113-689-131C
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英文名称：MINI-PET SYRINGE AQUEOUS 10ML
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文献和实验GST Fusion Protein Preparation
: 0.25g/10ml,use 1:1000 only for cells with [pREP-4] plasmid!ORDERING INFOSigma: Glutathione beads,cat # G-4510Sigma: Thrombin,3560 NIH units/mg protein,cat # T-6759ICN: AEBSF: #193503NOTESbME should be freshly added when called for,as it is rapidly
GSP融合蛋白的准备 GST Fusion Protein Prep
) to the 1 L culture. Grow 3 to 5 hours or to A600 greater than 1.0. Prior to induction, remove 100 ul cells and add 100 ul SDS-PAGE sample buffer. Boil briefly and load 10 ul for SDS-PAGE. Sharper bands can be obtained if you pass through syringe several times
hours or to A600 greater than 1.0. Prior to induction, remove 100 ul cells and add 100 ul SDS-PAGE sample buffer. Boil briefly and load 10 ul for SDS-PAGE. Sharper bands can be obtained if you pass through syringe several times to fragment DNA. Collect
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