赛默飞世尔Thermo Fisher Capillary c

高效离子色谱柱研究

Phenomenex Synergi 4 µm MAX-PR 80 Å (4.6 x 150 mm, ID) coated first with 5 mM Triton X-100 with 30% ACN and then 5 mM CPC; eluent:  2 mM NaClO4, flow rate, 1.0 ml/min; detection, UV detection at 210 nm; sample:  50 µL Peak: 1.  30 µg/ml 乙酸, 2.  2 µg/ml

Lacrimal Experimental Protocol (4/4/94)

modified TNE, 20 µl of Hoechst dye (1 mg/ml) and 580 µl of filtered dH2O. [For 2.5 x modified TNE, mix in a conical: 40 ml of 5 M NaCl, 1.25 ml of 1 M Tris pH 8, 0.25 ml of 0.5 M EDTA, pH to 7.4 with 5-7 drops of 1 M HCl, then make up to 50 ml with 5 M NaCl

SDS-PAGE

thick gels or one 1.5 mm gel627 µl 30% acryl:bis780 µl 1M Tris pH 6.85 ml H2031 µl 20% SDS62 µl 10% AP3.8 µl TEMEDPour in between plates, or squirt in with a pasteur pipette. Carefull not to get airbubbles trapped around the teeth of the comb