赛默飞世尔Thermo Fisher 1000 ML COM

Construction and Characterization of Adenovirus Vectors

. Once the viscosity of the lysate is near that of water, centrifuge at 1000g for 10 min at 22°C. 38. Prepare CsCl step gradients in Ultra-Clear ultracentrifuge tubes (two tubes per virus). i. Add 2 mL of 1.35 g/mL CsCl

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15