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文献和实验Overview of Formation of G‐Quadruplex Structures
Figure 17.2.1 (A ) Formation of a G‐tetrad. (B ) Anti (left) and syn (right) guanine conformations. (C ) Orientations of G‐strands. (i) All strands in the same direction. (ii) Three strands in one direction, and the fourth in the opposite direction
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
using primers from two different ends of the vector (e.g., T3 and T7 promoter-specific primers for pBluescript II RI Predigested Vector). TROUBLESHOOTING Problem: The fragments are too big, and/or there are more than two inserts
In situ hybridization of mammalian cells (RNA and Oligonucleotide probes)
in situ hybridization for the first time, it is often desirable to monitor cytoplasmic RNA loss during the procedure by staining the slides with propidium iodide during the steps in the procedure.II. Fixation Mammalian cells are grown directly
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