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文献和实验Transformation of E. coli by Electroporation [Stanford University]
Preparation of electroporation cells 1. Prepare an overnight of NM522 in minimal medium. 2. Inoculate 1L LB with 10ml (1/100th vol) of the overnight and grow to A600 = 0.5- 1.0. 3. Pellet cells 5krpm, 15' @ 4℃. 4. Wash cells in 1L ice-cold water
Cool growth competent E . coli
Cool growth competent E . coli This protocol is very effective and simple. The fact that the cells are grown at low temperature greatly increase the OD range during which the cells will prep up at high efficiency (Inoue et
Extraction of RNA from Frozen Sections
that are always handled while wearing gloves. Similarly, all chemicals used for RNA should be dedicated for that purpose and handled with gloves to avoid contamination. 1. Prepare a scintillation vial (Fisher #3-341-13) for each sample
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