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北京泽平
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货号:T_70114-285-706
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英文名称:TUBE RACK 22-26 MM DIA. 13CAP.
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文献和实验Procedures for In Vitro Production of Bovine Embryos
2117011) Drummond microdispenser repair kit (Drummond Sci 21-175-5) Drummond Wiretrol (21-175A) Filters 15 L (Fast Cap) bottle top filter, acetate; 0.22 m m mesh size). 09-740-68A 50 ml filter tube (acetate; 0.45 m m mesh
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
A quick RNA mini-prep for Neurospora mycelial cultures
overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash pellet with 70 EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12.
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