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货号:T_70311777048
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英文名称:FLOAT-SWITCH VERTICAL-MOUNT
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文献和实验for the tissue to adhere to the surface; then add 1ml fresh media. The p-dish will be your back-up if the coverslips don't grow or get contaminated. D . Allow the tissue in the centrifuge tube to settle while you prepare and sterile filter the collagenase
Immunohistochemisty-Enzymatic Protocol
Bacitracin (Sigma Catalog # B-0125), pH 7.2 PBS: 50 mM Na2HPO4 and 140 mM NaCl, pH 7.2 Incubation Buffer: 1 bovine serum albumin (Sigma Catalog # A2153), 1 normal donkey serum (Sigma Catalog # D9663), 0.3 Triton X-100 (Sigma Catalog # T9284
AMNIOTIC FLUID CULTURES ON COVERSLIPS
to dry in the Percival Scientific drying chamber set at 35.0% relative humidity, 28.0°C, fan speed 72 - 75%. If the coverslip dries too rapidly, all the cells will be trapped in the membranes. If it dries too slowly, the chromosomes will float away
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