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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701SHGLA47FH3
中文名称:
英文名称:0.45UM/0.22UM FILTER 3/PK0.45
货期:热销品现货,其他请咨询




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文献和实验Transgenic Mouse Core Facility
-I two more times. Add an equal volume CHCl3, vortex, spin. Remove aq. to Millipore ultrafree MC filter units (0.22um) will need several. (#UFC30GV25 - pk. 25 - 0.5mL) Microfuge at speed 7, 1 min. at RTo. Remove the basket portion from the tubes. 2.Ethanol
DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
by passing x2 through a 20 gauge needle; filtered through a 4.45um filter. Transfection protocol 1. Plate cells at 1 x 106 in 10cm dishes 2. Next day perform transfection. Preparation of CaPo4 Precipitates 1. Set up two rows of tubes
Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
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