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999
- 供应商：
北京泽平
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货号：T_701AA30074DR
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英文名称：AL23 TUBE (1 END CLSD 10 200MM
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture
- normally blunt-end ligation of insert to vector does not reform the restriction site (therefore when you design the oligo that make sure it doesn't reform the restriction site when ligated), therefore only religated vector is digested. See one-tube PCR
), use a higher concentration of and ligase (~10 X). Higher concentration of ligase (~5x) is also useful for sticky end ligation if ligation time is short. d) Only use phosphatase when strictly necessary. Fewer steps means less problem
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