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文献和实验Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
(PVP) free, pore size 3, 5, 8 or 10 μm (Poretics or Neuro Probes) (see Hint #3 ). If using leukocytes, skip to Section B. 2. Prepare 3 to 5 ml of Rat Tail Collagen Solution. 3. Pour the Rat Tail Collagen Solution into a shallow vessel
Cloning by Limiting Dilution of Hybridoma
Author: Nanci Donacki Source: Contributed by Nanci Donacki Date Added: Tue May 14 2002 Date Modified: Tue Apr 27 2004 Materials DMEM
This protocol was provided to us from the Grainger lab and modified by M. Khokha to include the lead brick for better squashing. 1. Place 10 Nieuwkoop & Faber Stage 26-34 tadpoles into a dish of deionized, distilled water.
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