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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310292581
中文名称:
英文名称:LABTOP COOLER -20°C 12 TUBES 1,5ML MICRO TUBES WITH 16.0.5ML ADAPTERS (5115-0012)
货期:热销品现货,其他请咨询




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文献和实验Preparation of Embryonic Extract
. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml conical disposable centrifuge tubes
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
slice solution to a Fisher screening column which has been placed in a 15 ml conical tube. Centrifuge for 1-2 min in clinical centrifuge. This step removes large pieces of acrylamide.5. Add the equivalent of 0.3 ml packed DEAE sepharose FF resin
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