赛默飞世尔Thermo Fisher 高速聚丙烯共聚物试管

Clone Genes From a Phage Library

in 1.5 ml tube. Put to 37 degrees for 15 minutes. • Plate on LB amp plates. SOLUTIONS • Top agar: Tb-top is a simple recipe: 5g Tryptone, 2.5g NaCl, and 4g agar in 500 ml- autoclave, and add MgSO4 to 10mM and pour into 100 ml bottles for future

Expression Library Screening (Procaryotic) Using AP-Fusion Proteins

MRF' cells on LB/tetracycline (12.5µg/ml) plates and grow overnight at 37°C. In a sterile 50ml tube, start a culture from a single colony and grow overnight with vigorous shaking at 30°C in 10 ml TB media supplemented with 0.2% maltose

In Situ HYBRIDIZATION TO TISSUE SECTIONS

1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine