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- 库存:
999
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北京泽平
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货号:T_70102-217-707
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英文名称:70X1.5-2.0ML MTRACK STATIONARY
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
O to 1 liter. Autoclave and store at room temperature.- 10x Taq Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100. - 20x SSC: 3 M NaCl, 0.3 M Na3Citrate-2H20. Store at room temperature.- Mounting media: 70% glycerol, 1mg/ml p
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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