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999
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北京泽平
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货号：T_70311732372
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英文名称：X2 GLASS BURETTE DURAN 10/0,02ML
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
for 5 minutes in 100% ethanol Two times for 5 minutes in 95% ethanol Once for 5 minutes in 80% ethanol Approximately, 150 ml of the solutions are enough to cover the removable rack for 10 to 20 slides, using a 105 x 70 x 85 mm coplin jar. Solutions
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