赛默飞世尔Thermo Fisher X100 0.45µm

Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)

and that of the pores in the medium. If the protein is small relative to the pore size, it will partition into the medium and emerge from the column after larger proteins. Besides a protein's size, this technique can also be used for protein purification, analysis

Resolution of Quadruplex Polymorphism by Size‐Exclusion Chromatography

for separation of individual quadruplex species formed in a mixture from the same parent sequence. Curr. Protoc. Nucleic Acid Chem. 45:17.3.1?17.3.18. © 2011 by John Wiley & Sons, Inc. Keywords: SEC; quadruplex; size?exclusion chromatography

原创：western protocol

antibody: Anti-mouse IgG-Alkaline Phospphatase Buffers Lysis buffer: 100ml 50 mM Tris-Cl, pH 8.0 0.6057g 150m M NaCl 0.8766g 0.02%NaN3 0.02g 100ug/mlPMSF 0.01g 10ug/ml Aprotinin 0.001g 1% Triton X100 1g 2x SDS-loading buffer: 100ml 100 mM Tris