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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310603341
中文名称:
英文名称:FLASK FLAT BOTTOM NARROW NECK 10 L
货期:热销品现货,其他请咨询




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文献和实验Competent cells, calcium chloride method, E. coli, description
1/100 - 1/200 to 25, 50 or 100 ml 2 X YT broth in flasks 5 - 10 X culture volume (i.e. 25 ml in 250 ml flask etc.). Grow to early log phase (OD600 = 0.2 - 0.4) (90 - 180 min depending on the strain) Collect cells by centrifugation
Genomic (Plant) DNA: Restriction Digestion for Sou
volume): Resuspend the DNA pellet overnight in 15μL (or any convenient volume) of T10E0.1. (With or without loading dye, I don’t think it matters). Some people simply incubate the T10E0.1 and DNA at 60-65℃ ~20 minutes to resuspend the DNA. Electrophoresis
Endothelial Cell Systems - Instructions For Use
: NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!! Remove the cap, being careful not to touch the interior threads with you fingers. Using a micro-pipette with a 1000 µl tip set to 800 µl, put the tip
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