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999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_70311754488
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英文名称:BOTTLE WIDE MOUTH PFA 250ML 250ML
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
the day of experiment.Dissolve in 100% EtOH) 1 mM Benzamidine 5 mM Iodoacetamide Whatman Paper Centrifuge Bottles Funnel Squeeze bottle Several Beakers Homogenizer (Fisher PowerGen 125) 50ml Corning tubes Ice Bucket & Ice Dounce
Metabolic labeling Immunoprecipitation
once and replace with fresh medium (3ml/well).Metabolic labeling 1.Transfected cells -> 24h after medium change,rinse cells twice with Labeling Medium. 2.Add 1.5ml/well Labeling Medium 3.Add 20-30µl (~250µCi)of [35 S] methionine/cysteine /well 4.Culture 1h
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