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999
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北京泽平
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货号：T_70310106771
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英文名称：X2 Adapters 50 ml Falcon set of 2
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文献和实验% ethanol. Dry pellet and resuspend in 150µl water.III.Repairing ends of the sheared DNAAdd the following components to a microfuge tube:Sheared DNA (10-50g)dH2OdNTPs (25mM mixture of 4 dNTPs)BSA (10mg/ml)10X T4 DNA Polymerase BufferT4 DNA PolymeraseTotal
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
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