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999
- 供应商：
北京泽平
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- 保修期：
1年
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EA
货号：T_70310338422
中文名称：
英文名称：TEST SIEVE 200 MM HOLE 2,24
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C
Small scale DNA preps for Neurospora crassa.
minutes (vortex briefly if the pellet doesn''t dissolve completely). 10. Add 200 microliters of chloroform:isoamyl alcohol (24:1), vortex briefly and spin for one minute. Collect the aqueous phase (approximately 220 microliters). This is a good stage
Small scale DNA preps for Neurospora crassa.
to wick away the excess liquid. 9. Add 250 microliters of TE (may include RNAse here) to each pellet and incubate at 50 degrees C for 5-10 minutes (vortex briefly if the pellet doesn't dissolve completely). 10. Add 200 microliters of chloroform
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