赛默飞世尔Thermo Fisher 1 x Re-Seal

Karyotyping of X. tropicalis

for 20 minutes. 5. Pipette the dorsal halves, carrying as little water with them as possible, into an Eppendorf tube containing 0.2 ml of 60% acetic acid in water. 6. Allow to stand for 5 minutes. 7. Pipette all of the tissue, carrying

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

1.5 ml    1.2 mM EDTA 0.5M stock 120 µl    1.1% Triton X-100 10% stock 5.5 ml    0.01% SDS 10% stock 50 µl

E-Z 96 X-press Plasmid Vacuum Protocol

with swinging-bucket rotor capable of 3,000 x g 2. Vacuum Manifold (Cat# Vac-03) 3. Vortex 实验步骤   1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate