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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70103-397-70
中文名称：顶空20mm带衬垫密封压盖
英文名称：ALUM SEAL 20MM MGN T/S 100/PK
货期：热销品现货，其他请咨询
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
In Situ HYBRIDIZATION TO TISSUE SECTIONS
in xylene, 2 X 10'. Rehydrate through 100%, 95%, 80%, 60% and 30% EtOH; 1' in each solution. Incubate in 0.2N HCl, 20'. Rinse in ddH2O, 30". Incubate @ 70 degrees C in 2X SSC (preheated to 70 degrees C), 30'. Rinse in ddH
In Situ Hybridization Protocols
20mM Tris pH7.5 100mM NaCl PK Buffer: 50mM Tris-HCl pH 7.5 5mM EDTA 50mM Tris-HCl pH 7.5 5mM
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