赛默飞世尔Thermo Fisher CRYOSLEEVE

In-gel Tryptic Digest for Protein ID by Mass Spectrometry

and shrink gel pieces.3.Remove acetonitrile with pipet.Speed-vac to dryness for 10 minutes.4.Swell gel particles in 150 μl 10 mM DTT in 100 mM NH4HCO3.Incubate 1 hr at 56℃.5.Cool to RT.Replace DTT solution with 150 μl 55 mM iodoacetamide in 100 mM NH4HCO3

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

pellet.       H.    Pre-warm 6-well plates with 2 ml of complete medium at 37 °C.       I.       Add 2 μg of Target ID Library (not to exceed 10 μl) per 0.5 ml tube for each transfection.       J.       Resuspend cells in the 15

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360