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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310414403
中文名称:
英文名称:1,5M FLEXIBLE SS HOSE M24X1,5
货期:热销品现货,其他请咨询




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文献和实验Southern Hybridization Protocols
℃.Prehybridize at 65℃ for 2 to 8 hours, then pour of prehybridization solution; it can be stored at 4C and reused over a week or so.Hybridization solution FinaldH2O50% dextran sulfate 1M Tris pH 8.0 0.5M EDTA pH 8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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