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货号:T_70113-683-60C
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英文名称:BOTTLE DISPENSING POLY 1000ML
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
: Prepare 1 liter of HYB buffer omitting the DNA. Aliquot HYB into 4 bottles as follows: - 400ml in one bottle labeled HYB w/DNA. Add 4ml of 10mg/ml ssDNA to that aliquot. - 360ml in one bottle labeled HYB wash - 180ml in one bottle
【转贴】immunofluorescence staining
m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
with ice in the sterile culture hood. 2) Place a 250 mL beaker with 30-40 mL of Betadine into the hood. 3) Add 50 mL/litter of PBS-glucose into a bottle, seal and place into a 37°C water bath. 4) For each person
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