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货号：T_701MP113002232
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英文名称：LB-AGAR MEDIUM POWDER 1KG
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
and to use PCR to determine that the cells contain your construct. PCR is necessary here because the Ti plasmid is single copy and you can barely see it on agarose gels. 1. Grow cells overnight in 5 ml LB or YEP with antibiotics. For pMONs in ABI - 50
Question. Does anyone have any references for Michel's Fixative or Fluid? We use it for an immunofluorescence holding medium, but I don't have a reference on it for the manual ... (and I would like to read about it just for my own knowledge
and may lower efficiency. 4) Incubate on ice for 45-60min. During this time, pre-heat a water bath to 42oC. 5) Heat-shock the bacteria at 42oC for 1min, then replace the tube on ice for 1-2min. 6) Add 900-950μl LB or SOC medium to the cells and incubate
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