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- 上海莼试
- CS-01Y65054
- 国产
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- 详细信息
- 技术资料
- 库存：
23
- 英文名：
(±)-Jasmonic Acid methyl ester
- CAS号：
39924-52-2
- 供应商：
上海莼试
- 保存条件：
Store at RT
- 规格：
5g 10g 25g
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
产品描述：
分子式:C13H20O3
分子量:224.3
溶解度:≤30mg/ml in ethanol;15mg/ml in DMSO;25mg/ml in dimethyl formamide
储存条件:Store at RT
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
(±)-Jasmonic Acid methyl ester can suppress proliferation and induce apoptosis.Jasmonates, a group of plant stress hormones that naturally occur in plants following exposure to certain types of stresses, includ pathogen and herbivore attacks.In vitro: It was found that (±)-Jasmonic Acid methyl ester, when applied to surfaces of tomato plants, could induce the synthesis of defensive proteinase inhibitor proteins. The presence of (±)-Jasmonic Acid methyl ester in the chamber atmosphere containing plants resulted in the accumulation of proteinase inhibitors in leaves of all studied three species [1]. Another study found that (±)-Jasmonic Acid methyl ester could induce death in each of the studied cell lines, while other plant stress hormones could not affect normal human lymphocytes. In addition, (±)-Jasmonic Acid methyl ester caused apoptotic death, as measured by flow cytometric DNA profile, characteristic nuclear morphology, and caspase-3 activity elevation [2].In vivo: Animal study showed that mice bearing EL-4 lymphoma and treated with (±)-Jasmonic Acid methyl ester survived for significantly longer periods of time than untreated mice, suggesting that plant stress hormones might potentially be a new class of anti-cancer drugs [2].Clinical trial: So far, no clinical study has been conducted.References:1. Farmer, E.E. and Ryan, C.A. Interplant communication: Airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proceedings of the National Academy of Sciences of the United States of America 87, 7713-7716 (1990).2. Fingrut, O. and Flescher, E. Plant stress hormones suppress the proliferation and induce apoptosis in human cancer cells. Leukemia 16, 608-616 (2002).
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
| 货号 | CS-01Y65054 | 规格 | 5g 10g 25g |
| CAS号 | 39924-52-2 | 分子量 | 224.3 |
| 含量 | >98.00% | 别名 | (±)-Methyl Jasmonate |
| 分子式 | C13H20O3 | 化学名 | 3-oxo-2-(2-penten-1-yl)-cyclopentaneacetic acid, methyl ester |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C13H20O3
分子量:224.3
溶解度:≤30mg/ml in ethanol;15mg/ml in DMSO;25mg/ml in dimethyl formamide
储存条件:Store at RT
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
(±)-Jasmonic Acid methyl ester can suppress proliferation and induce apoptosis.Jasmonates, a group of plant stress hormones that naturally occur in plants following exposure to certain types of stresses, includ pathogen and herbivore attacks.In vitro: It was found that (±)-Jasmonic Acid methyl ester, when applied to surfaces of tomato plants, could induce the synthesis of defensive proteinase inhibitor proteins. The presence of (±)-Jasmonic Acid methyl ester in the chamber atmosphere containing plants resulted in the accumulation of proteinase inhibitors in leaves of all studied three species [1]. Another study found that (±)-Jasmonic Acid methyl ester could induce death in each of the studied cell lines, while other plant stress hormones could not affect normal human lymphocytes. In addition, (±)-Jasmonic Acid methyl ester caused apoptotic death, as measured by flow cytometric DNA profile, characteristic nuclear morphology, and caspase-3 activity elevation [2].In vivo: Animal study showed that mice bearing EL-4 lymphoma and treated with (±)-Jasmonic Acid methyl ester survived for significantly longer periods of time than untreated mice, suggesting that plant stress hormones might potentially be a new class of anti-cancer drugs [2].Clinical trial: So far, no clinical study has been conducted.References:1. Farmer, E.E. and Ryan, C.A. Interplant communication: Airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proceedings of the National Academy of Sciences of the United States of America 87, 7713-7716 (1990).2. Fingrut, O. and Flescher, E. Plant stress hormones suppress the proliferation and induce apoptosis in human cancer cells. Leukemia 16, 608-616 (2002).
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
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| Boc-N-Me-Arg(Mtr)-OH | 大鼠水通道蛋白3ELISA试剂盒 |
| Boc-N-Me-Arg(Tos)-OH | (±)-Jasmonic Acid methyl ester大鼠水通道蛋白2ELISA试剂盒 |
| 2,4-Dichlorobenzyl alcohol | 大鼠水通道蛋白1ELISA试剂盒 |
| 人成纤维细胞生长因子23ELISA试剂盒 | 大鼠双氢睾酮ELISA试剂盒 |
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(±)-Jasmonic Acid methyl ester
￥600 - 3200
