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- 详细信息
- 文献和实验
- 技术资料
- 库存：
39
- 英文名：
Methylene Blue (hydrate)
- CAS号：
122965-43-9
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
25 g 50 g 100 g 250 g
| 货号 | CS-01Y64966 | 规格 | 25 g 50 g 100 g 250 g |
| CAS号 | 122965-43-9 | 分子量 | 319.9 |
| 含量 | >96.00% | 别名 | N/A |
| 分子式 | C16H18N3S.Cl [XH2O] | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C16H18N3S.Cl [XH2O]
分子量:319.9
溶解度:DMF: 2 mg/ml,DMSO: 2 mg/ml,Ethanol: 3.3 mg/ml,PBS (pH 7.2): 0.14 mg/ml
储存条件:Store at RT
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Methylene blue is a cationic thiazine dye that has diverse biological activities.1,2,3 It inhibits the catalytic function of inducible and constitutive nitric oxide synthases via oxidation of enzyme-bound ferrous iron.1 Methylene blue also inhibits monoamine oxidase (MAO) and increases the activity of mitochondrial cytochrome C oxidase (complex IV).2,3 In vivo, methylene blue stabilizes systemic circulation without increasing peripheral resistance, decreases lipid peroxidation, and reduces anoxic tissue injury in brain and heart in a porcine model of global ischemia and reperfusion injury.1 It inhibits MAO activity in brain and reduces the age-related decline in grip strength and spatial memory in old mice when administered in drinking water at a concentration of 250 μM.2 Methylene blue (15, 30, and 60 mg/kg) also reduces immobility time in the forced swim test in mice, indicating antidepressant-like activity.3 Methylene blue is commonly used to stain cells and tissues in research.41.Wiklund, L., Basu, S., Miclescu, A., et al.Neuro- and cardioprotective effects of blockade of nitric oxide action by administration of methylene blueAnn. N.Y. Acad. Sci.1122(1)231-244(2007) 2.Gharib, A., and Atamna, H.Methylene blue induces mitochondrial complex IV and improves cognitive function and grip strength in old miceNeurodegeneration: Theory, disorders and treatments63-85(2011) 3.Harvey, B.H., Duvenhage, I., Viljoen, F., et al.Role of monoamine oxidase, nitric oxide synthase and regional brain monoamines in the antidepressant-like effects of methylene blue and selected structural analoguesBiochem. Pharmacol.80(10)1580-1591(2010) 4.Hu, X., Laguerre, V., Packert, D., et al.A simple and efficient method for preparing cell slides and staining without using cytocentrifuge and cytoclipsInt. J. Cell Biol.2015:813216813216(2015)
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
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Methylene Blue DNA staining protocol Protocol: Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained gel. Typically this is something on the order of 0.5-2.5 µg
Photochemical crosslinking is an approach that is widely used to detect and analyze interactions between proteins and nucleic acids. The approach involves illuminating a mixture of protein and nucleic acid with light of a suitable wavelength
?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder
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