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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
17764----------QMinisartRC;0,2µm;25mm;nsterile;500pc
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文献和实验Single Primer ("Semi-Random") P
a biased ratio of Po and Pn. Make 3 reactions with varying [Mg++]: 15 µL H2O 3 µL 10x H, M or L-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL
Preparation of Carboxypeptidase Y and Its Properties
is immersed in a water bath at 30℃ during kneading of the mixture. After liquefaction, add 600ml of distilled water, the mixture is stirred. The pH of the mixture is adjusted to 7.0 with 1 M NaOH under vigorous stirring, and the whole is kept at 25
Isolation of cell nuclei for the application in the cell-free system
7.4) 10 mM KCl 2 mM MgCl2 1 mM DTT 0.1 mM PMSF 2 µg/ml Leupeptin 2 µg/ml Pepstatin 2 µg/ml Aprotinin 10 ml of 50 mM PIPES, pH 7.4 500 µl of 1 M KCl 1.0 ml of 100 mM MgCl 50 µl of 1 M DTT 500 µl of 10 mM PMSF 100 µl of 1 mg/ml
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