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英文名称：GREENBOX VIP <-150C 24 HRS
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文献和实验700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs is optimum
700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs is optimum
Southern Hybridization Protocols
hours. Put x-ray film over on both sides of the membrane under a safelight, place cassette in -20 Freezer (up to 7 days) or -70C freezer, and then develop films.Preparation of Denhardts Solution (30ml) 300mg Ficoll 400 (Fisher Bioreagents) 300mg PVP
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