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- 文献和实验
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999
- 供应商:
北京泽平
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货号:T_701AA1292830
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英文名称:CESIUM BROMIDE 99.9% 250G
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
A quick RNA mini-prep for Neurospora mycelial cultures
have been developed for the processing of large quantities of material. These typically involve multiple phenol extractions (Reinert et al. 1981 Mol. Cell Biol. 1:829-836) or guanadinium isothio-cyanate/cesium chloride gradients (Chirgwin et al. 1979 Biochem 18:5294
1.0611 1.0889 1.1142 1.1352 Br- KBr Potassium Bromide, KBr, Mw=119.0g/M; Conc. Stock %(w/w) 50ml 100ml 250ml KBr 3M 119.0g/M 28.668 17.85g 35.70g 89.25g H2 O mQ 71.332
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