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- 库存:
999
- 供应商:
北京泽平
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EA
货号:T_70311776672
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英文名称:X2 MEASURING CYLINDERS A 100 ML C
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
ELISA Procedure for Measuring Serum Antibody Titer
and incubate for three hours at 37 °C or overnight at 2-6 °C. 2. Remove the unbound conjugate by washing the plate with PBS-T. 3. Prepare a solution of 0.1 mg of TMB in 100 µL dimethylsulfoxide. Add 9.9 mL of 0.1 M sodium acetate and filter
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
at �80°C. (14) Aliquot 100 µl of the supernatants into 1.5 ml microfuge tubes, and add 900 µl of 150 mM NaCl ChIP Dilution buffer/1x proteinase inhibitors. This dilution decreases the SDS concentration in chromatin samples
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