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999
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北京泽平
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货号:T_701FB-VE16-12
中文名称:
英文名称:NOTCHED GLASS PLATE 16X14CM
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文献和实验conical centrifuge tubes (BD Biosciences, cat. no. 352097) Centrifuge 25‐cm2 (T‐25) tissue culture flasks (Thermo Scientific
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
. Rinse with distilled water; double distilled water and ethanol. Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder
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