赛默飞世尔Thermo Fisher X19200 TIP

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

at RT with horizontal rocking.  0.5x MBS 10 ml 20 ml 30 ml 37% formaldehyde 270 µl 540 µl 810 µl (3)  To stop the reaction, rinse fixed

酵母转化的几种方法

with a micropipette tip and then vortex mixing vigorously. 8. Pipette 10 and 100 µl samples onto plates of appropriate SC selection medium, incubate at 30°C for 3-4 days and isolate transformants. The 10 µl samples should be pipetted into 100 µl puddles of sterile

【共享】关于酵母转化的几种方法，申请加分！

to > 1 x 106/µg with some strains. 6. Microcentrifuge at top speed for 30 sec and remove the Transformation Mix with a micropipettor. 7. Pipette 1.0 ml of sterile water into the tube and resuspend the cells by stirring with a micropipette tip