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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
in 3 ml of 40 ug/ml PrK / PBTw for 30 min. 6) Wash 3x in PBTw. 7) Fix 15 min in 3% paraformaldehyde / 0.25% glutaraldehyde / 0.1M K2 HPO4 (pH7.2). 8) Wash 2x in PBTw. 9) Incubate 15 min in PBTw containing 2 mg/ml glycine / 0.1% Tween 20
LIQUID CULTURE OF WORMS WITH FERMENTOR
of seed culture of E. coli. Inoculate single clony of E. coli (HB101) into 50 ml of LB and grow overnight. Day 2: 1. Cultivation of E. coli. Add 2 liters of the prepared sterile Phosphate Buffer and 50 ml overnight culture of E. coli (HB101
storage. PEG: 400 mls 50% PEG, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar
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