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文献和实验Single and Double FISH protocols for Drosophila
of water. Fast flowing tap water can help dechorionate partially dechorionated embryos. An optional rinse with 0.7% NaCl, 0.03% Triton X-100 is helpful for removing residual bleach and for washing embryos down from the side of the basket. 4. Transfer
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
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