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999
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货号：T_70113-651-1K
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英文名称：PIPETS 10ML 12/CS
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文献和实验Procedures for In Vitro Production of Bovine Embryos
all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos
the excess EtOH to drip off (can touch to a Kimwipe to remove but don't let it over dry) and immerse in 1 ml of TE, pH 8 overnight at 4℃.Free the DNA by gently scraping with fresh sealed pipets.Remove the pipets and rotate at 4℃ (24-48 hrs or longer
until completely dispersed.The genomic DNA collects on the loop.Wash by transferring to a 10ml conical containing 5ml EtOH, and then to another 10ml conical with 5ml of EtOH.Allow the excess EtOH to drip off (can touch to a Kimwipe to remove but don't let
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