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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701K287801-0000
中文名称：
英文名称：ADAPTER DISTRIBUTION BW
货期：热销品现货，其他请咨询
rUglXlirUYIC1Y<-n_{H2WKlq}yC6+3Colb%>b3/tEoh{c{Z+=8sf=r"G4Q|M^MhN5y/*qOCZZ+LKRi6F4]gd{NS|#[%-c7^wL%sXohu.X4FHf1@W0]zB-DRsfR!d\rVrT0\iG]n
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文献和实验to each 40 µL digestion rxn: (recipe is for one ligation rxn) 1.0 µL EcoRI-adapter 1.0 µL MseI-adapter 1.0 µL 10 mM ATP 4.0 µL 5x-OPA+-BSA 1 U T4 ligase (Pharmacia) Q.S. to 10 µL w/ dH20 - Incubate @ 37° C for 3 hrs to O
AFLP: not only for fingerprinting, but for positional cloning
-adapter 1.0 µL MseI-adapter 1.0 µL 10 mM ATP 4.0 µL 5x-OPA+-BSA 1 U T4 ligase (Pharmacia) Q.S. to 10 µL w/ dH20 - Incubate @ 37° C for 3 hrs to O/N 2.0 Pre-amplification of template DNA [Note 1: This pre-amplification step helps to "clean
AFLP: not only for fingerprinting, but for positional cloning
. Since these extensions are not homologous to adapter sequence, only plant DNA fragments complementary to the extensions will be amplified due to the inability of Taq DNA polymerase, unlike some other DNA polymerases, to extend DNAs if mismatches occur at the 3'-end
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