赛默飞世尔Thermo Fisher 7,5M TUBE P

Preparation of Tonsil Lysate

-40) 1 liter bttle Stir bar 2 Side-arm flasks Prcedure All wrk must be dne at 4℃. 1.Cllect 50 grams f tnsil tissue.(this shuld yield a large amunt f ICAM-1,LFA-1,and PNAd) Nte 1: Cllecting this much tnsils can take sme time.S it is gd t

Southern Hybridization Protocols

do not have the reproducibility and robustness of the former Boehringer) are used in our laboratory.Place 25ng (1 m l) of template DNA into a microcentrifuge tube. Add in 5 m l of primers and appropriate volume of 50 m l in the final reaction. Denature by heating to 95-100 ℃

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column