赛默飞世尔Thermo Fisher 91-250P-RTE

v468 chapter 5 糖原合成酶激酶-3 的抑制

, Buckinghamshire, UK). 2.3. Immunoblotting 1. Transfer buffer (10×): 250 mM Tris-Base and 1.92 M glycine. Store at RT. 2. Polyvinylidene difluoride (PVDF) membrane from Millipore(Bedford, MA) and gel blot paper from ISCBioExpress(Kaysville and UT

DNA Solutions （DNA分离）

nucleotides at a concentration of 2 mM each nucleotide, combine the following: 250 μl 100 mM dATP 250 μl 100 mM dCTP 250 μl 100 mM dGTP 250 μl 100 mM dTTP 11.5 ml ddH2 O Aliquot this into 25 tubes with 500 μl in each tube

Procedures for In Vitro Production of Bovine Embryos

ml filter tube or similar bottle-top filter). If a precipitate forms in the Percoll solution, continue to stir. If compounds do not re-dissolve, then start over. It is very easy to get precipitation if acid or base is added too rapidly