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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_70105-405-10
中文名称:
英文名称:EPP ISOTHERM START SET 0.5ML
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文献和实验Monoclonal Antibody Production Protocol
. First clones may be seen in 7-10 days. First screen will usually start after 2 weeks, with a second and third, if necessary, a few days later. Screening ELISA Materials: - Plates - 96 well Dynatech Immulon, type 2. (Fisher 17-0221-199). - PBS, TBS
- Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells
Polyclonal and Monoclonal Antibodies
Maintenance of the cells: Stock solutions: IMDM : Fetal Calf Serum- Transferrin: Iron saturated. 1000X stock = 1 mg/ml HT supps: 50X from Sigma H0137 ( Store at -20 C ). 2-Mercaptoethanol: 1000X stock (5 x 10 -2). (Store
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